Cell Line Generation Protocol

Our xtencho tm cell line together with our in house xten protocol is your best chance at overcoming your difficult to express protein challenge.

Cell line generation protocol.

Infection of human b cells with ebv in vitro results in their immortalization and the resulting cell lines are named lymphoblastoid cell lines lcls in these cells ebv establishes mainly a latent infection characterized by the expression of a limited number of viral proteins. Atcc protocol was followed for subculturing. For optimal results we recommend following the cell culture recommendations of the supplier. Identify single clones by limited dilution.

Transfect cells with desired plasmid construct s select and expand stable polyclonal colonies. The protocol for generating stable cell lines requires several steps as shown below. This protocol is specific for the generation of a monoclonal cell line that resistance to antibiotics g418 neomycin. For stable cell line generation services vectorbuilder defines estimated turnaround as the time from production initiation to completion.

This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Epstein barr virus ebv is a lymphotropic γ herpes virus. Turnaround time does not include waiting time for any customer supplied materials e g. This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Stable cell line generation protocol. This is usually the first step before moving to stable cell line development in order to be able to ensure that all antibody features meet the requirements in cho based productions. After thawing from liquid nitrogen sw480 cells were maintained in nunclon delta t25 cell culture flasks in gibco dmem medium supplemented with 10 gibco fbs and 1 pen strep for 2 passages before seeding for spheroid generation. Generate a kill curve to determine the optimal selection antibiotic concentration.

Unlike the short term protein expression observed using transient transfection approaches generating cell lines using lentiviral vectors enables long term protein expression studies. The end result that you are looking for is a population of cells in which 100 of cells are expressing your fusion protein. Maintenance of cell line before spheroid generation. Production completion is achieved once the final cell line passes qc and is ready to be shipped out.