Cell Line Development Workflow

Cell line development is the process of establishing a clonally derived cell population which has been genetically engineered to express a desirable phenotype such as producing large amounts of recombinant protein for a stable period of time.

Cell line development workflow.

In cell line development cld you are looking for the rare high producing clones. Solentim technology streamlines workflows in the creation isolation and characterization of high value cells in cell line development vaccine production cell and gene therapy. We accelerate cell line workflow timelines from upstream development through scale up to production of new biological therapies. Interested in rapid and efficient stable cell pool cell line development for large scale production of your recombinant antibodies and proteins.

Cell line development cld on the beacon optofluidic system enables selection of the best production cell lines with 99 monoclonality assurance in just days. In the case of monoclonal antibody production the process of developing stable cell lines starts with transfecting host cells with recombinant plasmid dna encoding the. From vector design and host cell selection prior to transfection single cell cloning and colony outgrowth right through to the establishment of the master cell bank solentim technologies provide the evidence for. The opto cld workflow screens thousands of clones with ease in less than a week.

Typical cell line workflow. For the greatest genetic diversity you need to screen thousands of cells. Based on the suretechnology platform and unique world class expertise selexis sure cell line development services significantly reduce the time effort and costs that are associated with the development of high performance mammalian cell lines for therapeutic protein production e g development of monoclonal antibodies bi specific monoclonal antibodies growth factors and enzymes. Cell line development campaigns are typically performed over the course of several weeks to months at a time.

This innovative offering is based on uga s well established platform technologies including their proprietary cho host cell lines system optimized expression vectors and. The new opto cld 2 0 workflow extends these capabilities to allow 1 enrichment of rare cell populations 2 on chip titer measurements for traditional and non traditional antibody molecules and 3 efficient recovery. Single cells proliferate to form colonies that can then be assessed for.