Dish diameter actual area calculated area relative to 35 mm dish 35 mm 10 cm2 9 6 cm2 1 60 mm 220 cm 28 cm2 2 100 mm 60 cm 2 78 5 cm 6 actual area. Total flask area cm2 cell yield ml flask volume ml 25 cm2 25 2 5 x 106 5 7 5 70 rectangular 75 cm2 75 7 5 x 106 15 22 5 265 u shaped 150 cm2 150 1 5 x 107 30 45 377 u shaped 175 cm2 175 1 75 x 107 35 52 5 513 u shaped 225 cm2 225 2 25 x 107 45 67 5 1 006 traditional. Recommended growth average medium volume approx.
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